Optimal conditons are best of course. You will rarely if ever get to toxic NO2 levels in seawater. Reason, the NO2 has to compete with the chloride ion, which is 19,000 ppm, which acts as protection ion and to some extent so does Ca++. That is why "salt" is added to FW tanks when there is an NO2 problem, it lowers nitrite toxicity. In seawater, it is not lest toxic it is many, many times less toxic than, so it is not a concern but I still want it zero.