DSB's can go through a mini cycle at first with some anoxic build up if it is not stirred up at all. Mine did for about the first three months. Had a lot of black patches accumulate, lots of gassing, but with frequent stirring to release all the junk it settled down. No science behind this statement, but I believe it can take a while for bacteria to fully poplulate a deeper bed if it's not stirred at first. Never seemed to have a real effect on my livestock though.