Paul, your "gravel" would be 2 to 5mm in dia. ( most likely ) when you put it in. Dolomite if I remember correctly. This material, if used exclusively, in a freshly set up tank would not work. At the stated "low flow" of 150 gph in your 100 gal. tank, the gravel would be highly oxygenated, ALL OF IT !
The vertical flow rate at those parameters is 9/16" per minute. That flow will highly oxygenate the water in the substrate and not allow faculative bacteria to process Nitrate. You have a goodly amount of sand and even "mud" in your gravel, as you have stated, and I think the substrate would not "denitrify" without it.
"Blockages" from the sand, and the mud, and the rocks, and the bottles and chain, and whatever else, are causing channeling in your substrate IMO. Without this channeling from the various blockages, there would not be any Hypoxic areas in the bed, and Nitrate processing would be little to none.
You also have ozonated skimming which is quite a bit of help, and your algae is also sucking up a good bit of Nitrate and P as well. I'm sure you would agree on these two.
I know you think that you don't have much channeling, and I just can't agree there. The sand and mud in your tank, along with mineral detritus from over the years, is surely mixed into your gravel, and having much the same effect as the "fine substrate" that I propose. In fact it is probably much finer, since I insist the substrate is properly sifted or graded to avoid "fines".
I DO NOT PROPOSE SAND ! SAND is too "non-descript", as we have been through already. I told you all that grain size would be a bit more "sticky" to define. Surely enough "sand" and grain size have become the "sticky points" of this proposed methodology.
I will state this again, I think that "short circuiting", or "channeling" is probably the best way to reliably create the Hypoxic zones that we are looking for.
This channeling can be created using different grain sizes in either mound shaped or vertical "channels" of larger grains to promote flow, and smaller grain sizes in between these "channels" to reduce flow, leaving Hypoxic zones in between.
Silica sand would be preferred here for most of the bed, not including the upper layer, in order to remain stable, by not dissolving and to resist clumping.
Still, thorough straining is required to eliminate "fines". I'm not sure just what grades of grain size are available in Silica, or Quartz.
What appears to be a better solution currently, is the option of using industrial glass beads made primarily from silica or soda lime. These beads are for all intents and purposes, Inert, they are glass. The beads are available in very tight grain size ranges compared to "sand", and do not have any "fines" at all. None, nada.
This is what will allow a RUGF, and sand bed to be set-up and become a denitrifying bed in a short ( similar to DSB ) period of time. Paul's tank took many years to become appropriate for Reef animals thru the addition of everything under the sun, and that is not such a repeatable methodology, even though it continues to work very well for him.
The best grain size for the denitrification areas in the bed is still the paramater that I haven't quite zeroed in on exactly. My guess is that .3 to .6mm ( .012 to .024" ) would work very well. Some might want to include some smaller grains for the bacteria, but I'm not convinced yet. This is about 7 times larger than the "fines" that are included as optimum, by Ron Shimek, for "sand".
I need a source of information on this that is not induced by Shimek. You got any other leads Boomer ?
Stating that it won't work is just closed mindedness, come on people, it will work if you want it to and do the work to design it properly. I am doing that, and IT WILL WORK. The most interesting comment thus far has been from LiveforPhysics, regarding the relationship between Nitrate and P. I have to think on that some more to be sure.
With puposely made "channels" throughout the bed, the flow could be increased a lot if you wanted to, and the Hypoxic zones would still be there, albeit a little bit smaller.
The top 2" should still be what the animals like most, and pleasing to the eye. I guess something between .5 to 1.5mm, or 1 to 2mm Araganite is probably the best choice there.
What I'm proposing here has not been tried before to the best of my knowledge, and has not been disproven to say the least.
> Barry