I started with 30lbs of LR, about 30lbs of live sand from my 20 long that is now my sump, also used all of the water from my 20. I then added 50lbs of dry rock, 50lbs of new/dry substrate and a bottle of bacteria(dont remember the name, but it smells really bad). Now on week 3 and amonia, nitrites are 0 and nitrates are showing although low. This is with live stock in tank(very little).